Mouse trophoblast stem cells

Accession number BBBC033 · Version 1

Example images

  • example image
  • example image

Description of the biological application

Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered. Mouse trophoblast stem cells image contains a clustered monolayer of nuclei that can be difficult to segment. This image set is provided along with manually annotated ground truth that can be used for image analysis software testing purposes.

Images

Images acquired by using PerkinElmer Ultraview VoX spinning disk microscope combined with a Leica SP8 with a 63x immersion objective (Distance between Z-slices = 0.5 um)

  • 488 channel (C0): WGA
  • 405 channel (C2): Hoechst
  • Width = 103.424 microns (1024)
  • Height = 135.744 microns (1344)
  • Depth = 16 microns (32)
  • BBBC033_v1_dataset.zip (65.5 MB)

    Ground truth

    Ground truth 3D image contains manually annotated and segmented nuclei

    BBBC033DatasetGroundTruth.tif (88.1 MB)

    For more information

    These images were generated by the Laboratory for Modeling Development and Regeneration at Hubrecht Institute, The Netherlands. Please contact Nicolas Rivron and/or Javier Frias Aldeguer for more information.

    Published results using this image set

    The proposed data set will be evaluated in a publication to be submitted.

    Recommended citation

    "We used image set BBBC033v1 Rivron et al. 2018, available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]."

    Copyright

    CC0 To the extent possible under law, Nicolas Rivron has waived all copyright and related or neighboring rights to BBBC033v1.

    This page last updated 2018-03-05