Accession number BBBC015 · Version 1
The images are of a human osteosarcoma cell line (U2OS) co-expressing beta2 (b2AR) adrenergic receptor and arrestin-GFP protein molecules. The receptor was modified-type that generates "vesicle-type" spots upon ligand stimulation.
The plate was acquired on iCyte imaging cytometer with iCyte software version 2.5.1. Image file format is JPEG with one image for green channel and one image for crimson channel. Image size is 1000 x 768 pixels.
This image set has a portion of a 96-well plate containing 3 replica rows and 12 concentration points of isoproterenol. In each well four fields were acquired. File name structure: <well-number>_<field>_<channel>.JPG
The following file encodes this plate map in the format expected by CellProfiler's LoadText module:
|0.69*||Carpenter et al., Genome Biol., 2006|
|0.63*||Logan and Carpenter, J. Biomol. Screening, 2010|
These images were originally gathered for Ilya Ravkin's Invitation to Participate in the Comparison of Image Analysis Algorithms for Intracellular Screening.