Accession number BBBC014 · Version 1
This 96-well plate has images of cytoplasm to nucleus translocation of the transcription factor NFκB in MCF7 (human breast adenocarcinoma cell line) and A549 (human alveolar basal epithelial) cells in response to TNFα concentration.
Images are at 10x objective magnification. The plate was acquired at Vitra Bioscience on the CellCard reader. For each well there is one field with two images: a nuclear counterstain (DAPI) image and a signal stain (FITC) image. Image size is 1360 x 1024 pixels. Images are in 8-bit BMP format.
File name structure is <channel>-<well-number>-<row>-<column>-<field>.BMP
The plate map is a standard 96-well plate, containing 12 concentration points in columns and 4 replica rows for each cell type.
The following files encodes this plate map in the format expected by CellProfiler's LoadText module:
These images were originally gathered for Ilya Ravkin's Invitation to Participate in the Comparison of Image Analysis Algorithms for Intracellular Screening.